Products

DESCRIPTION

Basic Info

Model NO.	BS2008
Certification	CE, ISO9001
Host	Rabbit/Mouse
Reactivity	Human, Mouse, Rat
Transport Package	Refrigerated Transport
Specification	100ug/1mg
Trademark	Bioworld
Origin	China
HS Code	300219000
Production Capacity	3000 Vials

Product Description

Catalog No.	BS2008
Product Name	FXR2 (E576) polyclonal antibody
Applications	WB IHC IF IP
Alternative Name	Fragile X mental retardation syndrome-related protein 2; FMR1L2; FXR2
Swiss-Prot	P51116
Host	Rabbit
Reactivity	Human,Mouse,Rat
Application_all	WB: 1:500~1:1000 IHC/IF: 1:50~1:200 IP: 1:10~1:100
Product	Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity	FXR2 (E576) polyclonal antibody detects endogenous levels of FXR2 protein.
BiowMW	~ 74 kDa
Note	For research use only, not for use in diagnostic procedure.
Immunogen	Synthetic peptide, corresponding to amino acids 550-600 of Human FXR2.

• 
  Western blot (WB) analysis of FXR2 (E576) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:K562 whole cell lysate(40ug) Lane3:H9C2 whole cell lysate(40ug) Lane4:MEF whole cell lysate(40ug)
• 
  Immunohistochemistry (IHC) analyzes of FXR2 (E576) pAb in paraffin-embedded human colon carcinoma tissue at 1:50,showing cytoplasmic and nuclear staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
• 
  Immunoprecipitation of HEK293T cell lysate using FXR2 (E576) pAb (Sepharose Bead Conjugate) #BD0048(lane 2 and lane 3) .Lane 1 is 30% input. The western blot was probed using FXR2 (E576) #BS2008."↑"(supernatant); "↓(deposition)
• 
  Immunohistochemistry (IHC) analyzes of FXR2 (E576) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50,showing cytoplasmic and nuclear staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-R

Background

The FMR1 protein (or FMRP) is an RNA-binding protein that associates with polyribosomes and is a likely component of a messenger ribonuclear protein (mRNP) particle. It contains several features that are characteristics of RNA-binding proteins, including two hnRNPK homology (KH) domains and an RGG amino acid motif (RGG box). FMR1 can also interact with two fragile X syndrome related factors, FXR1 and FXR2, and these proteins form heterodimers through their N-terminal coilcoiled domains. FMR1 localizes to both the nucleus and the cytoplasm, and since it contains both a nuclear localization signal and a nuclear export signal it is also implicated in the nucleo-cytoplasmic transport of mRNAs.