Products

DESCRIPTION

Basic Info

Model NO.	BS1268
Certification	CE, ISO9001
Host	Rabbit/Mouse
Reactivity	Human, Mouse, Rat
Transport Package	Refrigerated Transport
Specification	100ug/1mg
Trademark	Bioworld
Origin	China
HS Code	300219000
Production Capacity	3000 Vials

Product Description

Catalog No.	BS1268
Product Name	p18 INK4c (D142) polyclonal antibody
Applications	WB IHC IP
Alternative Name	Cyclin-dependent kinase 4 inhibitor C; Cyclin-dependent kinase 6 inhibitor; p18-INK4c; p18-INK6; CDKN2C; CDKN6; p18INK4c; p18INK6; p18 INK6
Swiss-Prot	P42773
Host	Rabbit
Reactivity	Human,Mouse,Rat
Application_all	WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200
Product	Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity	p18 INK4c (D142) polyclonal antibody detects endogenous levels of p18 INK4c protein.
BiowMW	~ 18 kDa
Note	For research use only, not for use in diagnostic procedure.
Immunogen	Synthetic peptide, corresponding to amino acids 100-150 of Human p18 INK4c.

• 
  Western blot (WB) analysis of p18 INK4c (D142) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:Hela whole cell lysate(40ug) Lane4:CT26 whole cell lysate(40ug) Lane5:PC12 whole cell lysate(
• 
  Immunohistochemistry (IHC) analysis of p18 INK4c (D142) pAb in paraffin-embedded human breast carcinoma tissue
• 
  Immunoprecipitation of Hela cell lysate using p18 INK4c (D142) pAb (Sepharose Bead Conjugate) #BD0048(lane 2 and lane 3) .Lane 1 is 30% input. The western blot was probed using p18 INK4c (D142) #BS1268. "↑"(supernatant); "↓(deposition)

Background

The normal progression of cells through the cell cycle is under the control of the cyclin dependent protein kinases Cdk4 and Cdk6 which are subject to inhibition by the mitotic inhibitory protein p16. Isolated members of the p16 family have been designated p15 and p18, respectively. The p16 related protein, p18, interacts strongly with Cdk6 and to a lesser extent with Cdk4, but lacks apparent interaction with other Cdks. Recombinant p18 has been shown to inhibit cyclin D-Cdk6 kinase activity. In contrast to p21/p27 that form ternary complexes with cyclin-Cdks, only binary complexes of p15, p16 and p18 have been identified in association with Cdk4 and/or Cdk6.