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Sepapure FF Ni-Nta 5 Ml, for His-Tag Purification Column

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Sepapure FF Ni-Nta 5 Ml, for His-Tag Purification Column

Sepapure FF Ni-Nta 5 Ml, for His-Tag Purification Column

Sepapure FF Ni-NTA 5 ml, for His-tag purification Prepacked 5 ml, 100 µm, 3 bar The Sepapure ® FPLC column line offers h

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DESCRIPTION

Basic Info
Model NO. FF NI-NTA 5ml
After-sales Service Yes
Warranty 1 Year
Appliance The Analysis of Environmental Samples
Ray Type No
Gaspath System No
Detector UV
Energy Resolution N/a
Certification CE, ISO
Spectrometer Detector UV
Stationary Phase Solid Adsorbent
Mobile Phase Liquid
Chromatography Type Liquid Chromatography
Chromatographic Column Type Convetional Analytical Column
Chromatographic Column Length ID16*25mm
Chromatographic Column Diameter ID16*25mm
Separation Result Good Alternative
Recommend It to Any Lab Yes
Transport Package Wooden
Specification 200*80*80cm
Trademark Good Science
Origin China
HS Code 90272012
Production Capacity 200000 PCS Per Year
Product Description

Sepapure FF Ni-NTA 5 ml, for His-tag purification

Prepacked 5 ml, 100 µm, 3 bar

The Sepapure® FPLC column line offers high-quality alternatives for Size-Exclusion (SEC), Affinity (AC) and Ion-Exchange chromatography (IEX). The FPLC column line is completing the KNAUER FPLC portfolio and provides the best LC column addition to your FPLC system.

The FPLC columns come as prepacked ready-to-use 5 ml cartridge and can also be purchased as bulk resin. The media for affinity chromatography as well as ion-exchange chromatography is based on agarose, which is very proven in FPLC chro­matography.
For affinity chromatography the agarose solid phase is functionalized with ligands for purification of recombinantly expressed proteins with His- or GST-tags or antibodies via Protein A or Protein G affinity. The Ion-Exchange columns can be purchased as weak and strong anion or cation exchangers. The basis of our FPLC desalting column is dextran, which reliably separates small molecules and salts from valuable proteins.

With these materials, the Sepapure FPLC columns are the best matching bio­chromato­graphy columns to your precious FPLC system, giving you the complete and reliable FPLC package.

Sepapure FF Ni-NTA 5 ml, for His-tag purification

Prepacked 5 ml, 100 µm, 3 bar

Sepapure for desalting or bufferexchange of protein samples

ModificationNi-NTA
Special characteristicsfor His-tag purification
Base materialAgarose
Binding capacityn/a
Particle size100 µm
Size[mm]ID16*25
Pressure rating3 bar
ConnectionInlet 10 - 32 (1/16'') female / Outlet 10 - 32 (1/16'') male
Recommended pH range3 - 9

Pressure Rating :3bar
Binding capacity> 40 mg/ml

Sepapure FF Ni-Nta 5 Ml, for His-Tag Purification Column

Comparison of ion exchange columns

Ion exchangers are used in a variety of protein purification protocols. This application compares equivalent columns, a weak and a strong anion exchanger as well as a weak and a strong cation exchanger from two different vendors.
The columns were comparable in all assessed cases.

Sepapure FF Ni-Nta 5 Ml, for His-Tag Purification Column

Comparison of two column sets for antibody purification in an automated two step purification process

This application compares the automated purification of antibodies with the AZURA Bio-Two Step Purification System with different columns. Capacity and yield of the purified proteins were compared and revealed no significant differences in the performances of the two investigated column sets.

Sepapure FF Ni-Nta 5 Ml, for His-Tag Purification Column

Separation of proteins with anion exchange chromatography on Sepapure Q and DEAE

Ion exchange chromatography is one of the most widely used FPLC techniques for protein separation and purification. Depending on the charge of the sample and the resin cation or anion exchange chromatography is used. This application describes an easy separation of model proteins and explains how anion exchange chromatography works.

Separation of proteins with cation exchange chromatography on Sepapure SP and CM

Ion exchange chromatography is one of the most widely used FPLC techniques for protein separation and purification. Depending on the charge of the sample and the resin cation or anion exchange chromatography is used. This
application describes an easy separation of model proteins and explains how cation exchange chromatography works.


Size exclusion chromatography (SEC), separates molecules on the basis of size differences.

We offers desalting columns for group separations of large molecular weight biomolecules from small molecular weight compounds.


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